Put simply the 1st Law of thermodynamics says that total energy of the universe is constant, put another way energy cannot be created or destroyed, but it can change from. As a form of energy the same applies to matter.
From a creation perspective this makes sense since the Bible says the God completed his creation on day six. It is however a problem from an evolutionary perspective, since it needs Energy to naturally come from some place.
The Evolutionist solution is to invoke negative energy with the idea that they add up to 0 energy, but this concept is seriously flawed. Mathematically energy is:
E = fd
E = energy.
f = force.
d = distance over which the force acts over.
If the force is negative then the mass is pushed in a negative direction.
E = (-f)(-d) = fd
The result is that the energy is still positive. For energy to be negative the force would have to push the mass in the opposite direction from which it is applied.
E = f (-d)
The force of gravity is negative as a vector since it is always pulling a mass down. So the formula for gravitational force can best be written as:
g = gravitational force.
= mass 1.
= mass 2.
r = distance between the center of each mass.
G = the gravitational constant.
The potential energy ( P ) of a gravitational field is
obtained by integrating the force of gravity over the distance
from 
to 
.
= radius of
gravitating body.
= infinity.
Such that:
Since G, , and are all positive, P is also
positive. Since P = potential energy, the potential energy
of a gravitational field is positive and not negative.
This falsifies the idea that gravitational potential energy is
negative energy. Work as been done on the
affects of negative Energy, show that it would produce some affects
that
have not been observed in gravity.
The final nail the coffin of the notion that the total amount of
energy in
the universe is zero is that theoretically negative energy must be
followed by
a larger amount of positive energy so the positive energy must always
be larger
than negative energy. As a result the total energy of the universe must
be >
0.